Arthur Miller Essay Example for Free

Arthur Miller Essay The theme of morals and guilt is explored by the characterization of John Proctor. Proctor is portrayed as being around the age of thirty and a well-respected farmer in the towns community. He is described as the kind of man- powerful of body, even-tempered, and not easily led. From these descriptions, we recognize that he is a strong, calm individual who is quite cynical. As soon as Proctor appears in the play we see that he is very much in conflict with himself due to the terrible secret he conceals within the dark and forbidding chambers of his heart. A history of sin, which goes against every moral fibre of his body and the society in which he dwells. He is a sinner, a sinner not only against the moral fashion but against his own vision of decent conduct. John Proctor is guilty of committing adultery with the young Abigail Williams while she was serving as a servant in his household and this has a great effect on his conscience. Proctors conscience eats away at him throughout the play as it manifests itself into his relationship with people, especially his wife, Elizabeth. This is presented when Elizabeth states: I do not judge you. The magistrate sits in your heart that judges you. I never thought you but a good man, John- only somewhat bewildered. Here we see the idea of morality being reflected on a personal level, or Proctors conception of himself. He conceives himself as a blatant sinner because of his raw deeds, being his lechery. I blush for my sin, he admits to his wife. Overall, through the characterization of John Proctor the theme morals and guilt is presented and from this we see that his perception of right and wrong are decided by his own vision of decent conduct. Characterization is essential to the exploration and development of themes in The Crucible by Arthur Miller. The characterization of Rebecca Nurse explores the theme of evil. Abigail Williams explores the themes of evil as well as the power of fear and John Proctor explores the theme of morals and guilt. All these characters and many more help develop the themes within The Crucible.

Normalization of Genomic DNA Using Duplex-Specific Nuclease Essay

Whole genome shotgun sequencing (WGS) is an effective method for the study of reference sequences in genomes. It generates several sequences data, which result in overlapping sequences eventually. The aligning DNA sequences achieved overlapping sequence assembly into contigs that could read through the computer program. The WGS method is not applicable when redundant repetitive sequences exist in large genomes1 (cited in 1). Several methods such as methylation-spanning linker libraries (MSLL), Methylation filtration (MF) and others have used eradicating redundancy in higher plant genomes that depended on the hypermethylation tendency of repetitive sequences. The use of enzymes or a genomic library set up could modify the genome, but it is applicable to limited plant genomes 2-4 (cited in 1). The authors proposed another method in this article called ‘high-C0t DNA analysis’ that followed DNA renaturation kinetics in which sheared, denatured, and gradually reanneled genomi c DNA is used. Then, hydroxyapatite chromatography is used for separation of repetitive sequences (dsDNA) from low-copy sequences (ssDNA). With the help of detailed knowledge of DNA reassociation kinetics and advance skills in spectrophotometry, high-C0t DNA analysis can be applied to any genome5-7 (cited in 1). Shagina and others (2010) has discovered duplex-specific nuclease (DSN) normalization technology for genomic DNA (1). It is a simple method that based on hybridization kinetics excluding separation of both ssDNA and dsDNA. The authors’ isolated DSN enzyme from the Kamchatka crab that is thermostable and specific to dsDNA8 (cited in 1). They first denatured dsDNA that contained repetitive sequence and hydrolyzed it by DSN and then ran PCR on ssDNA (low-... ...tion of normalized cDNA libraries enriched with full-length sequences. Bioorganic Khim. 31:170-177. 10Zhulidov PA, Bogdanova EA, Shcheglov AS, Vagner LL, Khaspekov GL, Kozhemyako VB, Matz MV, Meleshkevitch E. (2004). Simple cDNA normalization using Kamchatka crab duplex-specific nuclease. Nucleic Acids Res 32:e37. 11Rodrigue S, Malmstrom RR, Berlin AM, Birren BW, Henn MR, and Chisholm SW. (2009). Whole genome amplification and de novo assembly of single bacterial cells. PLoS One 4:e6864. 12Cheung F, Haas BJ, Goldberg SM, May GD, Xiao Y, and Town CD. (2006). Sequencing Medicago truncatula expressed sequenced tags using 454 Life Sciences technology. BMC Genomics 7:272. 13Lander ES, Linton LM, Birren B, Nusbaum C, Zody MC, Baldwin J, Devon K, Dewar K. (2001). Initial sequencing and analysis of the human genome. Nature 409:860-921.

Battle of waterloo Essay

The Battle of Waterloo was fought thirteen kilometres south of Brussels between the French, under the command of Napoleon Bonaparte, and the Allied armies commanded by the Duke of Wellington from Britain and General Blucher from Prussia. The French defeat at Waterloo drew to a close 23 years of war beginning with the French Revolutionary wars in 1792 and continuing with the Napoleonic Wars from 1803. There was a brief eleven-month respite when Napoleon was forced to abdicate, exiled to the island of Elba. However, the unpopularity of Louis XVIII and the economic and social instability of France motivated him to return to Paris in March 1815. The Allies soon declared war once again. Napoleon’s final defeat at Waterloo marked the end of the Emperor’s final bid for power, the so-called ‘100 Days’, and the final chapter in his remarkable career. The Protagonists Napoleon Bonaparte had always been driven by his desire to make France a European empire and was an experienced warlord and leader. He had carried out a coup against the government of the First Republic of France (the ‘Directory’) in 1799 and established himself as ruler and First Consul, eventually to declare himself First Consul for life and Emperor in 1804. In 1802 the French Republic was officially ecognised and the Peace of Amiens signed. Napoleon had higher ambitions and pursued his desire to make France the most powerful country in Europe by conquering other countries including Britain. In 1803, Britain declared war on France and the ensuing ‘Napoleonic Wars’ were fought between France and various Allied coalitions over the next 1 1 years. The Allies successfully invaded in 1814 and forced Napoleon to abdicate at the Treaty of Fontainbleau. The European powers were meeting in Vienna to re-establish the territorial balance in Europe when news came of Napoleon’s escape from Elba on 1st March 181 5 and is re-entry into Paris on 20th March. The powers immediately renewed their declaration of war on Napoleon and the 7th Coalition between Britain, Austria, Prussia and Russia was formed on 25th March. They began assembling their troops in readiness for war, intending to attack along the French borders and march on Paris from different directions with enough strength to crush the French. In the event, only the armies of Wellington and Blucher were in place in Belgium. The Austrians and Russians arrived after Napoleon had been defeated. The Allied army under the Duke of Wellington was a coalition of British, Dutch, Belgian and German oldiers. Napoleon described Britain as ‘the most powerful and most constant of my enemies’. Arthur Wellesley, Duke of Wellington, had never been beaten by the French and had a reputation as a talented coalition general. He came to prominence in India and then successfully directed the Peninsular Campaign of 1811 when the British went to support Portugal and Spain against Napoleon. He was made a duke at the end of that war and appointed ambassador to the restored Bourbon court in 1814. Gebhard Leberecht von Blucher was the commander of the Prussian army. He was 72 at the time ot the Battle ot Waterloo and he only man to nave beaten Napoleon more than once. Age and experience meant that Blucher was less afraid of Napoleon than any other commander. His self-confidence and career record had a positive effect on his army, helping to keep morale amongst the Prussians high. Strategies After his return to France, Napoleon developed his strategy to defeat the Allies. He re-established himself in Paris and began building up his army in preparation for an invasion of Belgium, his goal was to capture Brussels. His battle plan was to mount an offensive attack on the Allied troops gathering in Belgium and to destroy them. In order to do this he wanted to divide the armies before defeating them separately, forcing Wellington’s army to retreat back to the Belgian coast in the west and the Prussians to retreat to the east. With speed he deployed his army along the French border and centred his headquarters at Beaumont Just across the border from Belgium. He was ready to attack on 15th June 1815. In order to separate Wellington and Blucher’s troops, Napoleon ordered Marshal Ney to advance on Quatre-Bras, a crossroads on the roads between Brussels and Charleroi and Nivelles and Namur. Allied Dutch-Belgium troops under Prince William of Orange were already positioned around the area and succeeded in holding off the French attack until reinforcements arrived. They continued to hold their ground, resulting in stalemate on 16th June. The result may have been a coincidence of timing. Had Marshal Ney attacked Quatre-Bras earlier the outcome could have been very different as, until the reinforcements arrived, the French army greatly outnumbered the Allies and their chances of victory were good. If the Allies had been defeated, they may have retreated completely rather than regrouping at Mont St Jean, a few kilometres north of Quatre-Bras. Wellington admitted surprise at the direction of the French attack; ‘Napoleon has humbugged me’, he declared. At Ligny, the Prussian army occupied strongholds of walled gardens, stone houses and farmhouses and lined up on the forward slope of the Ligny Brook with the right guarding the villages of St Armand and St Armand Haye. Heavvy fghting ensued with casualties on both sides, but the Prussians were forced to commit more and more troops. Although the French were victorious, they failed to totally destroy the Prussian army. They were able to retreat, albeit with numerous injured and dead, north to Wavre (about 18 kilometres east of Waterloo) Napoleon had succeeded in his aim of keeping the two Allied armies apart but mistakenly believed the Prussians were defeated. He was confident that Wellington could also be defeated. Preparing for battle On the morning of 18th June 181 5, Wellington was occupying the ridge of Mont St Jean, Just south of Waterloo, and Napoleon that of La Belle Alliance across the valley. The corps were divided into three under the commands of Lord Hill, Prince William of Orange and Sir Thomas Picton. Wellington was short of well-trained infantry and the cavalry were inexperienced, but he believed in the use of carefully deployed firepower. They had some 156 cannon and the standard gun for the infantry, a smoothbore mus et k titted with a bayonet on a socket over the barrel ot a gun Observing that his troops were outnumbered by the French, Wellington decided that his best plan was to stand firm until the Prussians could come to his aid.

Compare and Contrast between Angkor Wat and the Pyramid of...

Name: Tan Bunma Class: F2 Date: 12/10/2014 Compare and Contrast Pyramids of Giza and Angkor Wat â€Å"It is not the beauty of a building you should look at; it’s the construction of the foundation that will stand the test of time.† By David Allan Coe The quotation above means that the beauty of a building is not as important as the construction of the prototypes of its structure. Basically, the development of its foundations and techniques are more important than those of its attractiveness. Meanwhile, Angkor Wat and Pyramids of Giza are two ancient buildings that are able to continue its legacies to the contemporary world. Angkor Wat and the Pyramids of Giza are the examples of the external and internal beauties in which its structures were†¦show more content†¦Conversely, the authenticities of their designs are different. Particularly, Angkor Wat’s architectural designs are originated from the combination of the Indian’s culture and its neighboring cultures (UNESCO Angkor Wat, 2014). According to UNESCO (2014), â€Å"Khmer architecture evolved largely from that of the Indian subcontinent, from which it soon became clearly distinct as it developed its own special characteristics, some independently evolve d and others acquired from neighboring cultural traditions. The result was a new artistic horizon in oriental art and architecture.† On the contrary, Egyptian has developed its own (Beers, 1983, p. 23). Beers (1983, p. 24) has stated that â€Å"the Egyptians have improved hieroglyphics and developed the engineering skills needed to construct a more elaborate tombs for the pharaohs.† Although the Angkor Wat and the Pyramids of Giza located near big rivers and built with similar materials, their arts in architectural designs are originated differently. Even though Angkor Wat and the Pyramids of Giza were built in the period where their social developments are different, their citizens’ beliefs and suffering are following the same route. To begin with, Angkor Wat and Giza were built during different levels of development regarding its society. Angkor Wat was constructed during the